Dynamic FMR1 granule phase switch instructed by m6A modification contributes to maternal RNA decay
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ABSTRACT: To search for the functional “m6A readers” specifically in the context of Drosophila early embryos, we incubated m6A-modified or unmodified RNA probes with cytosolic lysates from early embryos, and performed immunoprecipitation experiments followed by mass spectrometry analysis. We found that Drosophila FMRP (FMR1) was highly abundant in complexes immuno-precipitated by the m6A-modified probe. We performed data-independent acquisition (DIA) quantitative proteomics on the wild type and fmr1 maternal mutant embryos at multiple developmental stages, including 0-1 h, 2-3 h, and 5-6 h stages. To screen the FMR1-associated partners, we collected the 2–3-hour embryos and performed immunoprecipitation using the anti-Drosophila FMR1 antibody, followed by mass spectrometric analysis.
ORGANISM(S): Drosophila Melanogaster
SUBMITTER: Dahua Chen
PROVIDER: PXD026356 | iProX | Sun Dec 19 00:00:00 GMT 2021
REPOSITORIES: iProX
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