Transcriptomics,Multiomics

Dataset Information

4

Transcription profiling by high throughput sequencing of mouse brain, heart, kidney, liver, lung, skeletal muscle organ, spleen and testis


ABSTRACT: As part of the ENCODE consortium the GENCODE project is producing a reference gene set through manual and automated gene prediction. Selected transcript models are verified experimentally by RT-PCR amplification of at least one of their unique splice junctions followed by high-throughput sequencing. The experiment targets are manually annotated transcripts with novel or putative status, non-pseudogene biotype and unique splice junctions which have not been validated previously and are not supported by mouse RNAseq data from the ENCODE project. In Batch M2, 808 splice junctions from GENCODE version M4 (released in December 2014) were chosen for experimental verification.

REANALYSIS of: E-MTAB-4644

ORGANISM(S): Mus musculus  

PROVIDER: E-MTAB-4644 | ExpressionAtlas | 2016-05-13

REPOSITORIES: ExpressionAtlas

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Publications


Within the ENCODE Consortium, GENCODE aimed to accurately annotate all protein-coding genes, pseudogenes, and noncoding transcribed loci in the human genome through manual curation and computational methods. Annotated transcript structures were assessed, and less well-supported loci were systematically, experimentally validated. Predicted exon-exon junctions were evaluated by RT-PCR amplification followed by highly multiplexed sequencing readout, a method we called RT-PCR-seq. Seventy-nine perce  ...[more]

Publication: 1/2

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