E-MTAB-5477
Ontology highlight
ABSTRACT:
PROVIDER: E-MTAB-5477 | ExpressionAtlas | 2019-05-14
REPOSITORIES: ExpressionAtlas
Similar Datasets
Project description:RNA-Seq of purified P-bodies from HEK293 cells
| PRJEB19388 | ENA
Project description:To identify RNAs accumulating in P-bodies (cytoslic RNP granules), P-bodies were purified from HEK293 cell extracts using a Flow Activated Particle Sorting (FAPS) method. Sorted P-bodies were compared to the pre-sorted fraction to identify RNA enriched in P-bodies.
2017-09-01 | E-MTAB-5477 | biostudies-arrayexpress
Project description:We report that solid-like condensates Amyloid-bodies are hubs of stress-resistant local nuclear translation in cells responding to high temperature. RNA-seq. analysis of purified Amyloid-bodies confirms that HSF1-transcribed mRNAs accumulate in these solid-like condensates during stress.
2021-01-15 | GSE164846 | GEO
Project description:To demonstrate RIPSeeker program that is developed for RIP-seq analyses, we generated RIP-seq data corresponding to the protein CCNT1 in HEK293 cell line using standard RIP-seq protocols described in Zhao et al., (2010). We performed two in-house RIP-seq experiments both for CCNT1 in human HEK293 cells. Briefly, we generated tagged CCNT1 using a triple tag system that supports lentiviral stable expression and mammalian affinity purification (MAPLE) Mak et al (2010). The HEK293 cells stably expressing tagged CCNT1 was purified by M2 agarose beads, followed by RNA extraction by Trizol. The library synthesis was carried out according to the RIP-seq protocol described in Zhao et al., (2010) except that one of the two experiments was done with non-strand-specific sequencing.
2012-12-28 | E-GEOD-43170 | biostudies-arrayexpress
Project description:This set of submissions contains the mass spectrometry files for the manuscript by Ji-Young Youn et al. that describes the high-density proximity mapping of RNA bodies. BioID experiments were performed from Flp-In T-REx HEK293 cells and MS files were acquired on Orbitrap Elite and Orbitrap Velos.
2017-08-04 | MSV000081411 | MassIVE
Project description:This set of submissions contains the mass spectrometry files for the manuscript by Ji-Young Youn et al. that describes the high-density proximity mapping of RNA bodies. BioID experiments were performed from Flp-In T-REx HEK293 cells and MS files were acquired on Orbitrap Elite and Orbitrap Velos. This submission is Set2 associated with SAINTexpress file ID3105.
2017-08-04 | MSV000081412 | MassIVE
Project description:RNA-seq of HEK293-TRDMT1-KD and HEK293 control
| PRJNA558168 | ENA
Project description:RNA-seq of 6 samples, 3 of them are TRDMT1 knockdown in HEK293 cells named HEK293-TRDMT1-KD and 3 of them are HEK293 control.
2022-07-07 | GSE135244 | GEO
Project description:small RNA seq form Hek 293 using IonTorrent Sequencing; To assess the general expression of snoRNA derived miRNA like fragments in Hek293. Three independent small RNA libraries were sequenced, aligned and subset for snoRNA (C/D, H/ACA, Small Cajal bodies); analysis of read-length for individual snoRNAs was used to define fragments, then to define coherent processing or degradation of the fragments the location of individual reads over the snoRNA annotation was define.
2019-09-04 | GSE136745 | GEO
Project description:We report here that human mitochondria contain small RNA including microRNA, piRNA, tRNA, rRNA, and RNA repeats. Mitochondria from human cells were purified and RNA isolated. Small RNAs were purified, library generated and analyzed by Illumina Hiseq 2000 system. The sequencing generated 19.5 and 17.7 million reads from HEK-293 and HeLa respectively. 91% and 97% sequences of HEK293 and HeLa respectively were annotated to various classes of small RNA. The total percentage of 4.21 and 2.58 sequences from HEK293 and HeLa respectively was found to be of miRNA. Further, we found only 1.2 % sequences from both the libraries aligned to mitochondrial genome. These results suggest that there is efficient transport of nuclear encoded small RNA to mitochondria. The small RNA in mitochondria may regulate critical cellular processes.
2013-03-05 | GSE32185 | GEO