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E-MTAB-8755

ABSTRACT: Effect of Tcf7l2 Knockout in the development and identity of Mouse brain Thalamus at embryonic stage E18.5 and P60

PROVIDER: E-MTAB-8755 | ExpressionAtlas | 2020-07-20

REPOSITORIES: ExpressionAtlas

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Effect of Tcf7l2 Knockout in the development and identity of Mouse brain Thalamus at embryonic stage E18.5 and P60

Project description:Effect of Tcf7l2 Knockout in the development and identity of Mouse brain Thalamus at embryonic stage E18.5 and P60

| PRJEB36527 | ENA

Effect of Tcf7l2 Knockout in the development and identity of Mouse brain Thalamus at embryonic stage E18.5 and P60

Project description:The thalamus of the brain acts as a relay station; taking inputs from several parts of the brain and then sending the information to the cortex and vice versa. It is also the structure know to affected in several brain developmental disorders such as schizophrenia, autism spectrum disorders, bipolar disorders etc. Upon in situ hybridisation one can visualise the expression of the transcription factor Tcf7l2 to be highest in prosomeric regions of the thalamus throughout development. With this information in mind we set out to find out, if the expression of Tcf7l2 is essential for the identity of the thalamic structure. Therefore, Tcf7l2 was knocked (KO) out using Cre+mice at embryonic stage E18.5 and postnatal adult stage P60. The E18.5 Tcf7l2 KO is a total knockout and the P60 Tcf7l2 KO is neuron-specific knockout. Total RNA was extracted and sent for sequencing using Illumina 2500. The data obtained was aligned by HISAT2 alignment tool, and the excon read counts were gathered using htseq counts, and expression normalization and differential gene expression was analysed using DESeq2.

2020-02-10 | E-MTAB-8755 | biostudies-arrayexpress

Illumina array to compare whole-genome expression change in miR-127 KO mice at E18.5 in 3 tissues: brain, skin and muscle

2014-05-20 | GSE47110 | GEO

Illumina array to compare whole-genome expression change in miR-127 KO mice at E18.5 in 3 tissues: brain, skin and muscle

Project description:miR-127 is an imprinted microRNA on mouse chromosome 12, strongly expressed during late embryogenesis and known regulator of placental gene Rtl1. miR-127-knockout (KO) mice appear phenotypically normal. An Illumina beadchip whole genome microarray experiment was carried out on embryonic stage 18.5 (E18.5) mice with a deletion in the miR-127 gene, and compared with wild type (WT) mice. Three tissues with varying expression of miR-127 were analysed: brain, skin and muscle. For each tissue (brain, skin, muscle) and genotype (WT or miR-127 KO), total RNA from 15 different embryos was extracted. These RNA samples were divided into three pools of five, to make three biological replicates. Each biological replicate was applied to two separate Illumina Mouse WG-6 v2.0 beadchips, to make two technical replicates.

2014-05-20 | E-GEOD-47110 | biostudies-arrayexpress

Roles of the kinase NUAK1 on spliceosome in the mouse cortex

Project description:The project focuses on the protein kinase NUAK1, which controls the development of brain cortical connectivity in mice. Recent data suggest that NUAK1 is involved in the regulation of the spliceosome and more broadly of mRNA biology. The aim of this project is to test whether inactivation of NUAK1 (constitutive KO) affects the expression level of genes relevant for cortical development in the embryonic brain. We collected the cortex of embryos (littermates) at the late embryonic developmental stage (E18.5, i.e. just before birth). The samples will be either KO or WT (controls). We collected the samples on the same day, under the same conditions, and as far as possible we made homogeneous groups: same litter, same proportion of males/females.

2024-01-22 | GSE226698 | GEO

Gene expression in the mouse embryonic small intestine in the presence or absence of E-cadherin

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Gene expression in the mouse embryonic small intestinal epithelium in the presence or absence of GATA4 and GATA6

Project description:Gene expression was compared between E18.5 Gata4Gata6 double conditional knockout (cKO) small intestinal epithelium and E18.5 control mouse small intestinal epithleium.

2014-04-30 | GSE49471 | GEO

Gene expression in the mouse embryonic small intestine in the presence or absence of E-cadherin

Project description:Gene expression was compared between E18.5 E-cadherin conditional knockout (cKO) small intestine and E18.5 control mouse small intestine. E18.5 mouse small intestine was collected from control and E-cadherin conditional knockout mice. RNA was prepared. Affymetrix Mouse Gene 1.0 gene arrays were used to interrogate gene expression. Data was analyzed using dChip software.

2012-02-28 | E-GEOD-31771 | biostudies-arrayexpress

Embryonic day 15 and Postnatal day 1 whole mouse brain AGTR2 knockout versus control

Project description:The gene expression profiles of control vs AGTR2 knockout mouse whole brains at developmental stage E15 and postnatal day 1 were examined. Keywords: genetic modification, brain development, AGTR2

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Gene expression in the mouse embryonic small intestinal epithelium in the presence or absence of GATA4 and GATA6

Project description:Gene expression was compared between E18.5 Gata4Gata6 double conditional knockout (cKO) small intestinal epithelium and E18.5 control mouse small intestinal epithleium. E18.5 mouse small intestine was collected from control and Gata4Gata6 double conditional knockout mice. Epithelial cells were isolated, and RNA was prepared. Affymetrix Mouse Gene 1.0 gene arrays were used to interrogate gene expression. Data was analyzed using dChip software.

2014-04-30 | E-GEOD-49471 | biostudies-arrayexpress

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