[Historical Versions Pending Deletion] Multiscale brain-wide mapping of α-synuclein-driven dopaminergic degeneration and white matter impairment in α-synucleinopathy mice

ABSTRACT: Citation: [Multi-modal imaging analysis of Parkinsonian mice reveals region-specific alterations in the substantia nigra] The spatiotemporal relationships among α-synuclein inclusion formation, dopaminergic degeneration, and white matter microstructural changes remain poorly defined. To address this, we unilaterally injected preformed fibrils (PFFs) or monomeric α-synuclein into the substantia nigra pars compacta (SNc) of wild-type mice. At 12 and 20 weeks post-injection (wpi), we performed ex vivo magnetic resonance imaging (MRI) at 9.4T to assess microstructural, volumetric and paramagnetic changes, along with light-sheet microscopy (LSM) to map α- synuclein aggregates, and dopaminergic neuron at single-cell resolution across the whole brain. We developed a Python-based, automated regis

SUBMITTER: Benjamin F. Combes 

PROVIDER: S-BIAD3402 | bioimages |

REPOSITORIES: bioimages