Electron tomography reveals mitochondrial network and cristae remodelling during cell differentiation in the human placenta (cryo-electron tomography)

ABSTRACT: Mitochondria adapt their structure through fusion and fission, yet how their morphology and cristae architecture change as cells differentiate remains unclear. The human placenta is a valuable model for studying mitochondrial diversity within a single tissue. As epithelial trophoblast cells of the placenta differentiate, their accompanying mitochondria morphologically and functionally transform. We used array tomography to characterise mitochondrial volume and network complexity. Cryo-electron tomography revealed two distinct subpopulations of mitochondria within progenitor cytotrophoblasts, and a singular, homogeneous population in the differentiated syncytiotrophoblast. We showcased the 3D topology of individual cristae through a standardised metric of “curvedness”. Proteomic analysis of

SUBMITTER: Siddharth Acharya 

PROVIDER: S-BIAD3409 | bioimages |

REPOSITORIES: bioimages