Systematic morphological profiling of human gene and allele function via Cell Painting (OME-NGFF)

ABSTRACT: OME-NGFF converted study from idr0033. We hypothesized that human genes and disease-associated alleles might be systematically functionally annotated using morphological profiling of cDNA constructs, via a microscopy-based Cell Painting assay. Indeed, 50% of the 220 tested genes yielded detectable morphological profiles, which grouped into biologically meaningful gene clusters consistent with known functional annotation (e.g., the RAS-RAF-MEK-ERK cascade). We used novel subpopulation-based visualization methods to interpret the morphological changes for specific clusters. This unbiased morphologic map of gene function revealed TRAF2/c-REL negative regulation of YAP1/WWTR1-responsive pathways. We confirmed this discovery of functional connectivity between the NF-kB pathway and Hippo pathwa

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PROVIDER: S-BIAD848 | bioimages |

SECONDARY ACCESSION(S): https://doi.org/10.17867/10000105

REPOSITORIES: bioimages