Circadian time series of dexamethasone synchronized U-2 OS knock-in cell expressing fluorescent fusion proteins of CRY1 and/or PER2

ABSTRACT: U-2 OS knock-in cells were genereated using CRISPR to express CRY1 and/or PER2 fused to either mScarlet-I or mClover3 from the endogenous promoter. Cells were synchronized with dexamethasone and imaged for 71h with an sampling interval of 1/h, starting 2 h after synchronization. Files are .nd2 and contain 3 channels: (1)RFP channel, (2)YFP channel, (3) DIC channel. All files are stacks with 71 timepoints. nd2 file image stacks can be opened using the freeware Fiji/ImageJ, but also contain alot of metadata. For each cell line, 10 imaging regions are provided. The cells identifiers are as follows: 42c5: CRY1-mClover3, 44c5: CRY1-mScarlet-I, 56c1: PER2-mClover3. 58b59: PER2-mScarlet-I. 80c16: CRY1-mClover3/PER2-mScarlet-I. Additionally, 4 imagiong areas without cells are provided, which were

ORGANISM(S): Homo sapiens (human)

SUBMITTER: Christian Gabriel 

PROVIDER: S-BSST630 | bioimages |

REPOSITORIES: bioimages