Dissection of the heat-shock response in Mycobacterium tuberculosis using mutants and microarrays

ABSTRACT: Regulation of the expression of heat-shock proteins plays an important role in the pathogenesis of Mycobacterium tuberculosis. The heat-shock response of bacteria involves genome-wide changes in gene expression. A combination of targeted mutagenesis and whole-genome expression profiling was used to characterize transcription factors responsible for control of genes encoding the major heat-shock proteins of M.tuberculosis. Two heat-shock regulons were identified. HspR acts as a transcriptional repressor for the members of the Hsp70 (DnaK) regulon, and HrcA similarly regulates the Hsp60 (GroE)response. These two specific repressor circuits overlap with broader transcriptional changes mediated by alternative sigma factors during exposure to high temperatures. Several previously undescribed he

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Graham Stewart 

PROVIDER: E-BUGS-2 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress