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Transcription profiling of dorsal pancreas tissue from Ngn3 null mice and wild-type littermates at days 13 and 15 of embryonic development

ABSTRACT: This experiment was designed to analyze the expression of genes in dorsal pancreatic cells at two temporally separated stages of pancreas development. This was accomplished by comparing expression profiles of embryonic dorsal pancreas tissue from Ngn3 null mice with wild-type littermates at days 13 and 15 of embryonic development. The comparison of gene expression in mutant and wild-type pancreas was used primarily to show genes that are lower expressed/missing in the mutant, as Ngn3 null mice have no endocrine pancreas tissue. From each developmental stage, five wild-type and five mutant samples were chosen, representing embryos from at least three different litters. Wild-type and mutant samples from the common stage of development were paired randomly and analysed in flipped colour. Probes were spotted in duplicate on each slide in a randomised (fixed) layout, effectively distributing the duplicate spots randomly over the slide.

ORGANISM(S): Mus musculus

SUBMITTER: Andreas Petri 

PROVIDER: E-CBIL-31 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The effect of neurogenin3 deficiency on pancreatic gene expression in embryonic mice.

Petri Andreas A   Ahnfelt-Rønne Jonas J   Frederiksen Klaus Stensgaard KS   Edwards David George DG   Madsen Dennis D   Serup Palle P   Fleckner Jan J   Heller R Scott RS  

Journal of molecular endocrinology 20061001 2

To understand the molecular mechanisms regulating pancreatic endocrine development and function, pancreatic gene expression was compared between Ngn3-deficient mice and littermate controls on embryonic days 13 and 15. Microarray analysis identified 504 genes with significant differences in expression. Fifty-two of these showed at least twofold reduction in Ngn3 knockouts compared to controls. Many of them were previously described to be involved in endocrine development and function. Among the g  ...[more]

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