High throughput decoding of anti trypanosomal drug efficacy and resistance

ABSTRACT: We used all five current human African trypanosomiasis drugs for genome-scale RNA interference (RNAi) target sequencing (RIT-seq) screens in Trypanosoma brucei, revealing the transporters, organelles, enzymes and metabolic pathways that function to facilitate anti-trypanosomal drug action. RIT-seq profiling links more than fifty additional genes to drug action. This data has been described in the following article [doi:10.1038/nature10771] and its further analysis can be freely submitted for publication. For information on the proper use of data shared by the Wellcome Trust Sanger Institute (including information on acknowledgement), please see http://www.sanger.ac.uk/datasharing/. Protocol - Assembly of the bloodstream-form T. brucei RNAi library and the RNAi target sequencing (RIT-seq) a

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Trypanosoma brucei

SUBMITTER:  

PROVIDER: E-ERAD-30 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress