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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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YPD to YPGal timecourse

ABSTRACT: Timecourse analyses (0 to 850 min) of exponentially growing BQS252 yeast after shift from YPD to YPGal galactose medium. Total RNA in vivo labeled by run-on, or cDNA labelling using random decamers included. Genomic DNA also examined. Keywords: other

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jose Perez-Ortin

PROVIDER: E-GEOD-1002 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genomic run-on evaluates transcription rates for all yeast genes and identifies gene regulatory mechanisms.

García-Martínez José J Aranda Agustín A Pérez-Ortín José E JE

Molecular cell 20040701 2

Most studies of eukaryotic gene regulation have been done looking at mature mRNA levels. Nevertheless, the steady-state mRNA level is the result of two opposing factors: transcription rate (TR) and mRNA degradation. Both can be important points to regulate gene expression. Here we show a new method that combines the use of nylon macroarrays and in vivo radioactive labeling of nascent RNA to quantify TRs, mRNA levels, and mRNA stabilities for all the S. cerevisiae genes. We found that during the ...[more]

PMID: 15260981

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