Transcription profiling of S. cerevisiae response to the antifungal compound sampangine

ABSTRACT: Sampangine, a plant-derived alkaloid found in the Annonaceae family, exhibits strong inhibitory activity against the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. In the present study, transcriptional profiling experiments coupled with the analysis of mutants were performed in an effort to elucidate its mechanism of action. Using Saccharomyces cerevisiae as a model organism, we show that sampangine produces a transcriptional response indicative of hypoxia, altering the expression of genes known to respond to low oxygen conditions. Several additional lines of evidence obtained suggest that these responses could involve effects on heme. First, the hem1 deletion mutant lacking the first enzyme in the heme biosynthetic pathway showed i

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Ameeta Agarwal 

PROVIDER: E-GEOD-10073 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress