Comparison of L. interrogans strains 56601 and IPAV at 37°C

ABSTRACT: DNA microarray analysis was used to compare the differential gene-expression profiles between Leptospira interrogans serovar Lai type strain 56601 and its corresponding attenuated strain IPAV. A 22 kb genomic island covering a cluster of 34 genes (LA0186 to LA0219) was actively expressed in both strains but concomitantly up-expressed in strain 56601 in contrast to that of IPAV. RT-PCR assays proved that the gene cluster comprised five transcripts. Gene annotation of this cluster revealed characteristics of a putative prophage-like remnant with at least 8 out of 34 CDSs encoding prophage-like proteins, of which, LA0195 is probably a putative prophage CI-like regulator. The transcription initiation activities of putative promoter-regulatory sequences of transcripts I, II and III, all proxima

ORGANISM(S): Leptospira interrogans serovar Lai str. 56601

SUBMITTER: Jin-Hong Qin 

PROVIDER: E-GEOD-10788 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress