Proteomics

Dataset Information

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Proteomic analysis of E. coli MG1655 after removal of oriC

ABSTRACT: The project aim was to describe proteomic changes in E. coli MG1655 strain after removal of oriC. Temperature-sensitive transcriptional repressor cI857 and its target promoter was used to control the expression of a serine recombinase from bacteriophage phiC31 (phiC31 integrase). cI857 is a temperature-sensitive mutant that represses its target promoter at 30°C, but the repression is relieved at 37°C. We generated an E. coli “switcher” strain in which the oriC sequence in the chromosome is flanked by phiC31-integrase-specific attP and attB sites and in this strain the oriC is irreversibly excised when cells are incubated at 37°C.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Villu Kasari  

LAB HEAD: Arvi Jõers

PROVIDER: PXD029931 | Pride | 2021-12-15

REPOSITORIES: Pride

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210308_03_Orbi2_KN_SER_G180_T210_K2rmas_1-83-2_2_25ug_iRT_150pg.raw Raw
210308_05_Orbi2_KN_SER_G180_T210_K2rmas_3-84-6_2_25ug_iRT_150pg.raw Raw
210308_07_Orbi2_KN_SER_G180_T210_K2rmas_3-83-3_2_25ug_iRT_150pg.raw Raw
210308_09_Orbi2_KN_SER_G180_T210_K2rmas_4-84-6_2_25ug_iRT_150pg.raw Raw
210308_11_Orbi2_KN_SER_G180_T210_K2rmas_1-84-6_2_25ug_iRT_150pg.raw Raw
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Publications

Decoupling Growth and Production by Removing the Origin of Replication from a Bacterial Chromosome.

Kasari Marje M   Kasari Villu V   Kärmas Mirjam M   Jõers Arvi A  

ACS synthetic biology 20220707 8

Efficient production of biochemicals and proteins in cell factories frequently benefits from a two-stage bioprocess in which growth and production phases are decoupled. Here, we describe a novel growth switch based on the permanent removal of the origin of replication (<i>oriC</i>) from the <i>Escherichia coli</i> chromosome. Without <i>oriC</i>, cells cannot initiate a new round of replication, and they stop growing while their metabolism remains active. Our system relies on a serine recombinas  ...[more]

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