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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Microarrays analysis of SREBP-1a and SREBP-1c target genes identify new regulatory pathways.

ABSTRACT: In this study we have identified the target genes of SREBP1a and SREBP1c in primary cultures of human skeletal muscle cells using adenoviral vectors expressing the mature nuclear form of human SREBP1a or SREBP1c combined with oligonucleotide microarrays. Keywords: comparison of human myotubes infected either by SREBP1a or 1C Human myotubes were prepared from 3 different skeletal muscle biopsies. After 7 days of differentiation, myotubes were infected for 48 hours with recombinant adenovirus expressing either Renilla, nuclear SREBP1a or nuclear SREBP1c. Each SREBP1a or SREBP1c infected myotubes culture was compared to Renilla infected myotubes culture. Renilla infected myotubes were considered as the control. Three biological replicates were processed.

ORGANISM(S): Homo sapiens

SUBMITTER: Jennifer Rieusset

PROVIDER: E-GEOD-10918 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle.

Rome Sophie S Lecomte Virginie V Meugnier Emmanuelle E Rieusset Jennifer J Debard Cyrille C Euthine Vanessa V Vidal Hubert H Lefai Etienne E

Physiological genomics 20080617 3

In this study we have identified the target genes of sterol regulatory element binding protein (SREBP)-1a and SREBP-1c in primary cultures of human skeletal muscle cells, using adenoviral vectors expressing the mature nuclear form of human SREBP-1a or SREBP-1c combined with oligonucleotide microarrays. Overexpression of SREBP-1a led to significant changes in the expression of 1,315 genes (655 upregulated and 660 downregulated), whereas overexpression of SREBP-1c modified the mRNA level of 514 ge ...[more]

PMID: 18559965

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Microarrays analysis of SREBP-1a and SREBP-1c target genes identify new regulatory pathways.

Project description:In this study we have identified the target genes of SREBP1a and SREBP1c in primary cultures of human skeletal muscle cells using adenoviral vectors expressing the mature nuclear form of human SREBP1a or SREBP1c combined with oligonucleotide microarrays. Keywords: comparison of human myotubes infected either by SREBP1a or 1C

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