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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Drosophila adult females Logjam p24 mutant to test the hypothesis that loss of loj function causes a transcriptional response characteristic of ER stress activation

ABSTRACT: We performed genome-wide profiling experiments on tissues from Drosophila melanogaster adult females with a mutation in the p24 gene logjam (loj) to test the hypothesis that loss of loj function causes a transcriptional response characteristic of ER stress activation. Experiment Overall Design: We compared expression profiles from loj mutant females to those of controls. We compared mutant and control abdomen tissue as well as mutant and control tissue preparations from the remainder of the fly (the head/thorax). Three mutant and three control RNA samples for each set of tissue were used for array hybridization (12 total array samples).

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Ginger Carney

PROVIDER: E-GEOD-10940 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Loss of p24 function in Drosophila melanogaster causes a stress response and increased levels of NF-kappaB-regulated gene products.

Boltz Kara A KA Carney Ginger E GE

BMC genomics 20080508

<h4>Background</h4>Secretory and transmembrane proteins traverse the endoplasmic reticulum (ER) and Golgi compartments for final maturation prior to reaching their functional destinations. Members of the p24 protein family, which are transmembrane constituents of ER and Golgi-derived transport vesicles, function in trafficking some secretory proteins in yeast and higher eukaryotes. Yeast p24 mutants have minor secretory defects and induce an ER stress response that likely results from accumulati ...[more]

PMID: 18466616

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