Sex of Blood

ABSTRACT: Blood samples were taken at 1:00 pm +/- 1 h (Potassium-EDTA-monovettes, Sarstedt, Numbrecht, Germany) to control for circadian influences on gene expression. Blood was taken from normal male controls, normal female controls as well as different patients with disorders of sex development leading to a dissection of sex chromosomes and external genital phenotype. All samples were analyzed by a blood cell counter (Beckmann-Coulter, Krefeld, Germany) and white blood cell counts did not differ between the normal males and the normal females (by t-test). PBMCs were isolated from whole blood using PBS (pH 7.2) and Ficoll (Biochrom, Berlin, Germany) gradient centrifugation. A reference experiement design type is where all samples are compared to a common reference. Disease State: diagnosis Genotype

ORGANISM(S): Homo sapiens

SUBMITTER: Paul-Martin Holterhus 

PROVIDER: E-GEOD-11235 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress