Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Sex of Blood

ABSTRACT: Blood samples were taken at 1:00 pm +/- 1 h (Potassium-EDTA-monovettes, Sarstedt, Numbrecht, Germany) to control for circadian influences on gene expression. Blood was taken from normal male controls, normal female controls as well as different patients with disorders of sex development leading to a dissection of sex chromosomes and external genital phenotype. All samples were analyzed by a blood cell counter (Beckmann-Coulter, Krefeld, Germany) and white blood cell counts did not differ between the normal males and the normal females (by t-test). PBMCs were isolated from whole blood using PBS (pH 7.2) and Ficoll (Biochrom, Berlin, Germany) gradient centrifugation. A reference experiement design type is where all samples are compared to a common reference. Disease State: diagnosis Genotype: sex genotype Age: age at blood sample collection (yrs;mo) Phenotype: external genitalia (Prader classification) Keywords: reference_design Computed

ORGANISM(S): Homo sapiens

SUBMITTER: Paul-Martin Holterhus

PROVIDER: E-GEOD-11235 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Disorders of sex development expose transcriptional autonomy of genetic sex and androgen-programmed hormonal sex in human blood leukocytes.

Holterhus Paul-Martin PM Bebermeier Jan-Hendrik JH Werner Ralf R Demeter Janos J Richter-Unruh Annette A Cario Gunnar G Appari Mahesh M Siebert Reiner R Riepe Felix F Brooks James D JD Hiort Olaf O

BMC genomics 20090701

<h4>Background</h4>Gender appears to be determined by independent programs controlled by the sex-chromosomes and by androgen-dependent programming during embryonic development. To enable experimental dissection of these components in the human, we performed genome-wide profiling of the transcriptomes of peripheral blood mononuclear cells (PBMC) in patients with rare defined "disorders of sex development" (DSD, e.g., 46, XY-females due to defective androgen biosynthesis) compared to normal 46, XY ...[more]

PMID: 19570224

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Project description:Throughout the course of early development, the reliable occurrence of stereotyped events is imperative. This careful orchestration is dependent upon regulation at the level of gene expression. While all cell types derive from a single fertilized cell and share a single genome, individual cell types differentially utilize this genome. Such differential utilization is essential for the acquisition, maintenance, and modulation of cell properties. In order to better understand the molecular basis of early development, we evaluated the mRNA expression programs of both early post-implantation mouse embryos and differentiating embryonic stem cells using a cDNA microarray platform covering 74% of the mouse genome. Mining of these datasets revealed a strong association across RNA processing, modulation of the cell cycle, and chromatin organization. Genes involved in these processes demonstrated sharp regulation during well-defined biological transitions. Dominant patterns of expression with biologically separable properties were identified. The evolutionary conservation of one cluster in particular suggested a molecular basis for the alignment of the murine and fly developmental programs. Moreover, the data suggest putative gene relationships that may also have broader implications for gene networks connecting the cell cycle to the molecular repertoire of the cell. Design: Dataset is a murine embryonic developmental timecourse consisting of morphologically staged samples from E6.25 to E9.0 (at approximately 0.25d intervals). There two replicates of each sample and there are 13 samples in each biological replicate series. A common reference design was employed. A development or differentiation experiment design type assays events associated with development or differentiation or moving through a life cycle. Development applies to organism(s) acquiring a mature state, and differentiation applies to cells acquiring specialized functions. Developmental Stage: morphologically staged samples from E6.25 to E9.0 (at approximately 0.25d intervals) Keywords: development_or_differentiation_design Using regression correlation

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Dataset Information

Sex of Blood

Publications

Disorders of sex development expose transcriptional autonomy of genetic sex and androgen-programmed hormonal sex in human blood leukocytes.

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