A single SR-like protein, Npl3, promotes pre-mRNA splicing in budding yeast

ABSTRACT: Mammalian SR proteins are a family of reversibly phosphorylated RNA binding proteins primarily studied for their roles in alternative splicing. While budding yeast lack alternative splicing, they do have three SR-like proteins: Npl3, Gbp2, and Hrb1. However, these have been primarily studied for their roles in mRNA export, leaving their potential roles in splicing largely unexplored. Here we combined high-density genetic interaction profiling and genome-wide splicing-sensitive microarray analysis to demonstrate that a single SR-like protein, Npl3, is required for efficient splicing of a large set of pre-mRNAs in Saccharomyces cerevisiae. We tested the hypothesis that Npl3 promotes splicing by facilitating co-transcriptional recruitment of splicing factors. Using chromatin immunoprecipitati

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Tracy Kress 

PROVIDER: E-GEOD-11491 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress