Trancription profiling of pig placenta, Erhualian pigs and Large White pigs, gestation day 75 and 90
Ontology highlight
ABSTRACT: From gestation day 75 to gestation day 90, an important stage for the placental and fetal development, the fetuses grow rapidly and need adequate nutrition. The Meishan pigs and the Large White pigs employ different ways in supplying the enough nutrients and oxygen to the fetus. The Meishan pigs increased the vascular density and the Large White pigs have the second increase in the surface of placenta. To understand the molecular basis related to late gestation placenta development in Chinese indigenous and Western breeds with different placental efficiency, samples were collected and used to hybridized. The results offered new data on understanding the molecular basis of placenta efficiency, and indicated that Erhualian pigs had the more efficient than the Large White pigs. Experiment Overall Design: Placenta efficiency (the body weight of a piglet divided by the mass of its placenta) of Erhualian pigs is markedly higher than Large White pigs. so placenta samples (female) from 6 Erhuanlian pregnant gilts at gestation day 75 (E75) and day 90 (E90) and 6 Large White pregnant gilts at gestation day 75 (L75) and day 90 (L90) were collected. RNAs from two female piglet placentas from each gilt were combined to 12 pools and hybridized to the porcine Affymetrix GeneChip.
Project description:From gestation day 75 to gestation day 90, an important stage for the placental and fetal development, the fetuses grow rapidly and need adequate nutrition. The Meishan pigs and the Large White pigs employ different ways in supplying the enough nutrients and oxygen to the fetus. The Meishan pigs increased the vascular density and the Large White pigs have the second increase in the surface of placenta. To understand the molecular basis related to late gestation placenta development in Chinese indigenous and Western breeds with different placental efficiency, samples were collected and used to hybridized. The results offered new data on understanding the molecular basis of placenta efficiency, and indicated that Erhualian pigs had the more efficient than the Large White pigs.
Project description:Background: The anterior pituitary plays a crucial role in endocrine regulation and homeostasis by orchestrating hormonal signaling, cellular growth, and functional adaptations. Adipokines regulate hypothalamic-pituitary-ovarian axis influencing hormone secretion and cellular processes. Omentin-1, an adipokine primarily expressed in visceral adipose tissue, is involved in metabolic and reproductive functions. However, its role in pituitary remains unknown. We hypothesize that omentin-1 modulates protein abundance in pituitary cells. This study aimed to investigate role of omentin-1 in regulating key pathways in anterior pituitary cells of pigs with different metabolic statuses: normal-weight Large White pigs and genetically obese Meishan pigs. Results: High-throughput proteomic analysis of porcine anterior pituitary cells exposed to omentin-1 revealed 230 differentially abundant proteins (30 downregulated and 3 upregulated in Large White pigs, as well as 107 downregulated and 90 upregulated in Meishan pigs), associated with 116 gene ontology terms. The most significantly altered proteins were associated with cell cycle and DNA replication, gene expression regulation and posttranscriptional/posttranslational modifications. Apoptotic and proliferative mechanisms varied between breeds, suggesting different metabolic adaptation strategies. In Large White pigs, CDK5RAP2 and SIX1 played a key role in transcriptional and proliferative regulation, whereas in Meishan pigs, stronger proliferative control was linked to AKT1S1 and RHOA. Moreover, Meishan pigs demonstrated a more dynamic regulation of apoptotic pathways (HTRA2, PARP2, DFFA), which may promote greater metabolic plasticity and better adaptation to physiological fluctuations. Additional in vitro experiments in Large White pigs showed that omentin-1 influenced key proliferative and apoptotic markers, downregulating cyclins D1, E1, A2, and B1, as well as caspase-3, while upregulating the anti-apoptotic protein BCL2, leading to an increased BCL2/BAX ratio. Further, omentin-1 modulated phosphorylation of selected kinases, including ERK1/2, AKT, AMPKα, and STAT3, in a time-dependent manner. It is also increased insulin receptor expression and promoted packaging and transport of secretory proteins. Conclusions: Proteomic and functional analyses revealed that omentin-1 regardless of breed exerts anti-proliferative and anti-apoptotic effects on porcine anterior pituitary cells, modulating key signaling pathways. These findings highlight that omentin-1 is a potential metabolic regulator influencing anterior pituitary function.
Project description:Affymetrix miRNA arrays were used to generate miRNA profiles in placenta of Meishan and Yorkshire pigs on stages of initiation (gestational day 26) and establishment (gestational day 50) of placental folds development. The study allowed for the determination of the miRNAs that were differentially expressed in porcine placenta on different gestational day. This study will provide the information to better understanding of the role miRNAs in porcine placental development.
Project description:The study allowed for the determination of the mRNAs that were differentially expressed in porcine endometrium on different gestational day and breed. The majority of the mRNAs were differentially expressed during the implantation stage. This study will provide the information to better understanding of the role of these differently expressed mRNAs in the porcine endometrium during pregnancy. Affymetrix mRNA arrays were used to generate mRNA profiles of on the endometrium of Meishan and Yorkshire pigs on days 15 (implantation stage), 26 (post-implantation stage) and 50 (mid-gestation stage) of gestation. The endometrium from different gestational day and breed were collected. In order to determine the mRNA expression profile of these tissue, RNA was extracted, labeled and hybridized on an Affymetrix mRNA array.
Project description:We collected the ovary samples at 49 days post coitus in Large White and Meishan adult female pigs, identified the differentially expressed protein coding genes and long non-coding RNAs between these two breeds. three individuals of each breed were harvested as biological replicates.
Project description:A CNV map in pigs could facilitate the identification of chromosomal regions that segregate for important economic and disease phenotypes. The goal of this study was to identify CNV regions (CNVRs) in pigs based on a custom array comparative genome hybridization (aCGH). We carried out a custom-made array comparative genome hybridization (aCGH) experiment in order to identify copy number variations (CNVs) in the pig genome analysing animals of diverse pig breeds (White Duroc, Yangxin, Erhualian, Tongcheng, Large White, Pietrain, Landrace and Chinese new pig line DIV ) using a tiling oligonucleotide array with ~720,000 probes designed on the pig genome (Sus scrofa genome version 9.0). In this study, a custom-made tiling oligo-nucleotide 720k array was used with a median probe spacing of 2506 bp for screening 12 pigs with a female Duroc as the reference. WD: White Duroc (♀); YX: Yangxin (♂); EH: Erhualian (♀); TC: Tongcheng (♀); LW: Large White (♀); PT: Pietrain (♂); LD1: Landrace × DIV pig 1 (♂); LD2: Landrace × DIV pig 2 (♀); DIV1: Chinese new pig line DIV 1 (♀); DIV2: Chinese new pig line DIV 2 (♀); L1: Landrace 1 (♂); L2: Landrace 2 (♂).
Project description:BACKGROUND: Litter size in pigs is a major factor affecting the profitability in the pig industry. The peri-implantation window in pigs is characterized by the coordinated interactions between the maternal uterine endometrium and the rapidly elongating conceptuses and represents a period of time during which a large percentage of the developing conceptuses are lost. However, the gene expression and regulatory networks in the endometrium contributing to the establishment of the maternal: placental interface remain poorly understood. RESULTS: We characterized the endometrial gene expression profile during the peri-implantation stage of development by comparing two breeds that demonstrate very different reproductive efficiencies. We employed the porcine Affymetrix GeneChip® to assay the transcriptomic profiles of genes expressed in the uterine endometrium obtained from Meishan and Yorkshire gilts (n = 4 for each breed) on day 12 of gestation (M12 and Y12, respectively). Total of 17,076 probesets were identified as "present" in at least two arrays. A mixed model-based statistical analysis predicted a total of 2,656 (q < 0.1) transcripts as differentially expressed between Meishan and Yorkshire pigs. Eighteen differentially expressed transcripts of interest were validated by quantitative real-time PCR. Gene ontology (GO) annotation revealed that the known functions of the differentially expressed genes were involved in a series of important biological processes relevant to early pregnancy establishment in the pig. CONCLUSIONS: The results identified endometrial gene expression profiles of two breeds differing in litter size and identified candidate genes that are related to known physiological pathways related to reproductive prolificacy. These findings provide a deeper understanding of molecular pathways differing between two breeds at the critical peri-implantation stage of pregnancy, which can be utilized to better understand the events contributing to pregnancy establishment in the pig. Meishan and Yorkshire breeds endometirum were selected and RNA been extracted and hybridized to the Affymetrix microarray. A number of genes have been shown differently expressed on two breeds.
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig