Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse rectum after physiological stress

ABSTRACT: Using isolation stress model, we showed that goblet cell-dependent mucosal barrier functions in the mouse rectum was more vulnerable to stress than the colon. We also found that isolation stress specifically stimulated IL-18 production only in the rectum. Furthermore, the crucial role of IL-18 in the stress response of the mouse rectum was confirmed using IL-18 knockout mice. Microarray analysis was used to assess the stress response and to identify responsible genes for the vulnerability of the mouse rectum to isolation stress. Experiment Overall Design: Total RNA was prepared from the colon and the rectum of wild-type and IL-18 knockout mice before isolation and 8 days after starting isolation. The one GSM sample of microarray analysis was made by pooling an equal amount of total RNA 5 mice of each group. Agilent whole mouse genome oligonucleotide microarray was used to measure gene expression in these samples according to the manufactureâ??s protocol.

ORGANISM(S): Mus musculus

SUBMITTER: Tomoko Kawai

PROVIDER: E-GEOD-11910 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Interleukin-18 is a crucial determinant of vulnerability of the mouse rectum to psychosocial stress.

Nishida Kensei K Kamizato Mai M Kawai Tomoko T Masuda Kiyoshi K Takeo Keiko K Teshima-Kondo Shigetada S Tanahashi Toshihito T Rokutan Kazuhito K

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20090113 6

Psychosocial factors are important determinants of disease manifestations, treatment efficacy, and prognosis of functional and inflammatory bowel disorders. Isolation of C57BL/6J mice from their 4 brothers growing in the same cage reduced goblet cells and MUC2 expression with a peak on day 8 in the rectum, but not in the colon. Gene expression analysis using a whole mouse genome microarray showed that the stress induced a 10-fold larger change in the gene expression in the rectum (722 genes) tha ...[more]

PMID: 19141531

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Dataset Information

Transcription profiling of mouse rectum after physiological stress

Publications

Interleukin-18 is a crucial determinant of vulnerability of the mouse rectum to psychosocial stress.

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