Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling by array of human hepatocytes treated with doxorubicin

ABSTRACT: A variety of important anticancer drugs kill cells by increasing cellular levels of topoisomerase II-DNA cleavage complex. The anthracycline anticancer drug doxorubicin forms a stable ternary complex with DNA and topoisomerase IIa, thereby inhibiting the normal function of the enzyme. In this study we found genes regulated by doxorubicin - induced and repressed - to be located much closer to each other than genes distributed randomly all over the genome (< 100 kbp). Experiment Overall Design: We calculated specifically the probability by which particular genes which were deregulated by the treatment of human hepatocytes with doxorubicin will occur within DNA windows of different sizes as compared to the probability of all known mapped genes (RefSeq transcripts) of the human genome (NCBI RefSeq 19,360; build 36.2) to occur within the same DNA windows.

ORGANISM(S): Homo sapiens

SUBMITTER: Juergen Borlak

PROVIDER: E-GEOD-11940 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Topoisomerase II inhibition involves characteristic chromosomal expression patterns.

Reymann Susanne S Borlak Jürgen J

BMC genomics 20080708

<h4>Background</h4>The phenomenon of co-localization of transcriptionally upregulated genes showing similar expression levels is known across all eukaryotic genomes. We recently mapped the Aroclor 1254-regulated transcriptome back onto the genome and provided evidence for the statistically significant co-localization of regulated genes. They did, however, not always show similar expression levels, and many of the regulated genes were, in fact, repressed.<h4>Results</h4>In this study, we were abl ...[more]

PMID: 18611269

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Dataset Information

Transcription profiling by array of human hepatocytes treated with doxorubicin

Publications

Topoisomerase II inhibition involves characteristic chromosomal expression patterns.

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