Gene expression profiling by DNA microarray in Colo741 cell line transfected by KRas-G12D or KRas-G12V

ABSTRACT: Mutations of the proto-oncogene KRas, together with the inactivation of the onco-suppressor genes APC and TP53, are considered to have an important role both in the carcinogenesis and in the colorectal tumor progression. The oncogene K-ras has activating mutations, especially in codon 12, in about 40% to 50% of colorectal carcinomas (Andreyev et al., 1998). The mechanism by which oncogenic K-ras alone contribuites to tumor progression in colon cancer is largely unknown. To this end, we have developed a model system where the colorectal adenocarcinoma cell line Colo741 has been stably trasfected with the Kras-G12V or Kras-G12D mutated oncogenes. Our results demonstrate how expression profiling data can be used to interpret changes in cellular characteristics induced by transfection and he

ORGANISM(S): Homo sapiens

SUBMITTER: Massimiliano Monticone 

PROVIDER: E-GEOD-12398 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress