Transcription profiling by array of human conjunctival epithelial side population cells

ABSTRACT: Side population (SP) cells isolated from limbal and conjunctival epithelia derive from cells that are slow cycling in vivo, a known feature of tissue stem cells. The purpose of this study was to define the molecular signature of the conjunctival side population cells by global differential gene expression to identify markers and signaling pathways associated with this cell phenotype. Four overnight cultures of freshly isolated human conjunctival epithelial cells stained with Hoechst 3342 were cytometrically sorted into SP and non-SP cohorts. RNA was isolated and processed for microarray analysis using a commercial oligonucleotide array representing more than 55,000 transcripts derived from about 30,000 different genes. Selected microarray results were validated at the gene and protein lev

ORGANISM(S): Homo sapiens

SUBMITTER: J Mario Wolosin 

PROVIDER: E-GEOD-12631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress