Macrophages stimulated with LPS and Poly (I:C)

ABSTRACT: The aim of the current study was to investigate the response of in vitro differentiated trout macrophages, a primary cell culture system widely used in fish immunology research, to lipopolysaccharide (LPS) and to the analog viral ds(RNA) Poly I:C. To that end we have used a salmonid-specific microarray platform enriched with immune-related genes. The results clearly suggest that the molecular mechanisms involved in the response of macrophages to LPS and Poly (I:C) are specific in some signaling pathways related to cell communication, signal transduction and kinase cascades. Nevertheless, macrophages stimulated with bacterial or viral PAMPs also activate common transcription factors. Trout macrophages were isolated using the protocol previously described Head kidneys were dissected, placed

ORGANISM(S): Oncorhynchus mykiss

SUBMITTER: Carmen Doñate 

PROVIDER: E-GEOD-13197 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress