Estimating accuracy of absolute gene expression measurement by RNA-Seq and microarrays with proteomics

ABSTRACT: Microarrays revolutionized biological research by enabling gene expression comparisons on a transcriptome-wide scale. Microarrays, however, do not estimate absolute expression level accurately. At present, high throughput sequencing is emerging as an alternative methodology for transcriptome studies. Although free of many limitations imposed by microarray design, its potential to estimate absolute transcript levels is unknown. In this study, we evaluate relative accuracy of microarrays and transcriptome sequencing (RNA-Seq) using third methodology: proteomics. We find that RNA-Seq provides a better estimate of absolute expression levels. We first determined whether we could reproduce the agreement between mRNA expression estimates measured by microarrays and by RNA-Seq reported in other st

ORGANISM(S): Homo sapiens

SUBMITTER: Xing Fu 

PROVIDER: E-GEOD-13744 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress