Transcription profiling of human CD3-positive T cells from SLE and controls reveals activation of mTOR controls the loss of TCRI? in lupus T cells through HRES-1/Rab4-regulated lysosomal degradation

ABSTRACT: CD3-positive T cells were negatively isolated from 10 SLE patients and 9 healthy controls without SLE. All of the SLE samples and control samples were compared with one another to identify baseline differences in expression due to the disease. Next, T cell preparations from 4 of the control subjects were stimulated with either Nitric Oxide (NOC-18) 600 uM for 24hr or stimulated through CD3/CD28 for 24hr to determine which genes were responsive to these signaling mechanisms. Here, we show that activity of the mammalian target of rapamycin (mTOR), which is a sensor of the mitochondrial transmembrane potential, is increased in SLE T cells. Activation of mTOR was inducible by NO, a key trigger of MHP which in turn enhanced the expression of HRES-1/Rab4, a small GTPase that regulates recyclin

ORGANISM(S): Homo sapiens

SUBMITTER: Frank Middleton 

PROVIDER: E-GEOD-13887 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress