Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Human epithelial cell response to bacterial signal indole

ABSTRACT: Indole is a bacterial signal secreted by pathogenic and commensal Escherichia coli in the stationary phase at high concentrations (~600 ÂµM). Prior work from our lab has shown that indole decreases E. coli O157:H7 (EHEC) chemotaxis, motility, attachment to epithelial cells and biofilm formation. However, its effect on epithelial cells is not known. We hypothesized that indole induces gene expression changes in epithelial cells that lead to decreased pathogen colonization and infection. Changes in gene expression with the human enterocyte cell line HCT-8 exposed to indole suggested down-regulation of Toll-like receptor signaling and coordinated changes in Jak-STAT and p38 MAPK pathways. Corresponding changes in the expression of cytokines, chemokines, and their receptors also suggested that indole functions as a modulator of inflammation in intestinal epithelial cells. In addition, the expression of genes involved in tight junction organization (claudins, and tight junction proteins) and mucin production were also up-regulated by indole. Keywords: Inter-kingdom signaling interactions between human cells and bacterial signal indole Human intestinal epithelial cells were exposed to 1 mM indole for 4 h or 24 h. RNA was isolated from the control cells and cells exposed to indole for 4 h and 24 h. The experiments were performed in triplicate. A total of 9 microarrays were performed, three for each condition. The gene expression data was analyzed using significance analysis of microarrays (SAM) where a false discovery rate cut-off of 1% was used. The genes were then classified and importnat regulated pathways were reported.

ORGANISM(S): Homo sapiens

SUBMITTER: Tarun Bansal

PROVIDER: E-GEOD-14379 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The bacterial signal indole increases epithelial-cell tight-junction resistance and attenuates indicators of inflammation.

Bansal Tarun T Alaniz Robert C RC Wood Thomas K TK Jayaraman Arul A

Proceedings of the National Academy of Sciences of the United States of America 20091204 1

Interkingdom signaling is established in the gastrointestinal tract in that human hormones trigger responses in bacteria; here, we show that the corollary is true, that a specific bacterial signal, indole, is recognized as a beneficial signal in intestinal epithelial cells. Our prior work has shown that indole, secreted by commensal Escherichia coli and detected in human feces, reduces pathogenic E. coli chemotaxis, motility, and attachment to epithelial cells. However, the effect of indole on i ...[more]

PMID: 19966295

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Dataset Information

Human epithelial cell response to bacterial signal indole

Publications

The bacterial signal indole increases epithelial-cell tight-junction resistance and attenuates indicators of inflammation.

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