RNA degradation in proliferating and differentiated C2C12 muscle precursor cells analyzed on Affymetrix exon arrays

ABSTRACT: Steady-state RNA levels are a result of RNA synthesis and degradation. The importance of transcription-factor mediated induction or repression of mRNA synthesis is well established, but the role and mechanisms of RNA degradation are less well understood. We globally evaluated the RNA decay rates in proliferating and differentiated mouse myoblasts on whole-genome Affymetrix exon arrays, allowing for the assessment of directionality of RNA degradation and the detection of splice variant-specific differences in RNA decay rates. We found large differences in decay rates. mRNAs coding for proteins involved in signal transduction and transcriptional regulation have shortest half lives, whereas mRNAs coding for DNA replication enzymes and muscle contraction proteins are among the most stable. Man

ORGANISM(S): Mus musculus

SUBMITTER: Peter 't Hoen 

PROVIDER: E-GEOD-14387 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress