Methylation orofiling of human stem cell reprogramming by targeted bisulfite sequencing

ABSTRACT: We report a method for specific capture of an arbitrary subset of genomic targets for single molecule bisulfite sequencing, and for digital quantitation of DNA methylation at a single nucleotide resolution. We used targeted bisulfite sequencing to characterize the changes of DNA methylation during the de-differentiation of human fibroblasts into hybrid stem cells, and into induced pluripotent stem cells. We compared the methylation level of approximately 66,000 CpG sites within 2020 CpG islands on chromosome 12, chromosome 20, and 34 selected regions. A total of 288 differentially methylated regions were identified between fibroblasts and pluripotent cells. Methylation cluster analysis revealed distinct methylation patterns between fibroblasts and pluripotent cells. Furthermore iPS cells a

ORGANISM(S): Homo sapiens

SUBMITTER: Kun Zhang 

PROVIDER: E-GEOD-15007 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress