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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Using high-density exon arrays to profile gene expression in closely related species (Exon 1.0 ST)

ABSTRACT: Global comparisons of gene expression profiles between species provide significant insight into gene regulation, evolutionary processes, and disease mechanisms. In this work, we describe a flexible and intuitive approach for global expression profiling of closely related species, using high-density exon arrays designed for a single reference genome. The high-density probe coverage of exon arrays allows us to select the identical set of perfect-match probes for measuring expression levels of orthologous genes. This eliminates a serious confounding factor in probe affinity effects of species-specific microarray probes, and enables direct comparisons of estimated expression indexes across species. Keywords: analysis of gene expression in tissues We conducted Human Exon 1.0 array profiling of RNAs from three chimpanzee cerebellums and three chimpanzee livers

ORGANISM(S): Pan troglodytes

SUBMITTER: Yi Xing

PROVIDER: E-GEOD-15626 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Using high-density exon arrays to profile gene expression in closely related species.

Lin Lan L Liu Song S Brockway Heather H Seok Junhee J Jiang Peng P Wong Wing Hung WH Xing Yi Y

Nucleic acids research 20090527 12

Global comparisons of gene expression profiles between species provide significant insight into gene regulation, evolutionary processes and disease mechanisms. In this work, we describe a flexible and intuitive approach for global expression profiling of closely related species, using high-density exon arrays designed for a single reference genome. The high-density probe coverage of exon arrays allows us to select identical sets of perfect-match probes to measure expression levels of orthologous ...[more]

PMID: 19474342

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Using high-density exon arrays to profile gene expression in closely related species (HJAY)

Project description:Global comparisons of gene expression profiles between species provide significant insight into gene regulation, evolutionary processes, and disease mechanisms. In this work, we describe a flexible and intuitive approach for global expression profiling of closely related species, using high-density exon arrays designed for a single reference genome. The high-density probe coverage of exon arrays allows us to select the identical set of perfect-match probes for measuring expression levels of orthologous genes. This eliminates a serious confounding factor in probe affinity effects of species-specific microarray probes, and enables direct comparisons of estimated expression indexes across species. Keywords: analysis of gene expression in tissues We conducted Affymetrix Human Exon Junction array (HJAY array) profiling of RNAs from human, chimpanzee and rhesus macaque cerebellums, with three replicates per species.

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Using high-density exon arrays to profile gene expression in closely related species

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Using high-density exon arrays to profile gene expression in closely related species (Exon 1.0 ST)

2009-05-05 | GSE15626 | GEO

Using high-density exon arrays to profile gene expression in closely related species (HJAY)

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A mutation in the Srrm4 gene causes pre-mRNA splicing defects and deafness in Bronx waltzer mice.

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