Transcription profiling by array of whole blood from sickle-cell disease patients using globin mRNA reduction

ABSTRACT: Room temperature whole blood mRNA stabilization procedures, such as the PAX gene system, are critical for the application of transcriptional analysis to population-based clinical studies. Global transcriptome analysis of whole blood RNA using microarrays has proven to be challenging due to the high abundance of globin transcripts that constitute 70% of whole blood mRNA in the blood. This is a particular problem in patients with sickle-cell disease, secondary to the high abundance of globin-expressing nucleated red blood cells and reticulocytes in the circulation . In order to more accurately measure the steady state whole blood transcriptome in sickle-cell patients, we evaluated the efficacy of reducing globin transcripts in PAXgene stabilized RNA samples for genome-wide transcriptome ana

ORGANISM(S): Homo sapiens

SUBMITTER: Xiuli Xu 

PROVIDER: E-GEOD-16728 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress