Gene Expression Data from Senescent vs Non-senescent Cells in the in Vivo Human Annulus

ABSTRACT: The presence of senescent cells in the aging/degenerating human disc is now well-recognized. Senescent cells are viable, cannot divide, remain metabolically active and accumulate within the disc over time. Molecular analysis of senescent cells in tissue, however, offers a special challenge since there are no cell surface markers for senescence which would let one use fluorescence-activated cell sorting as a method for separating out senescent cells. Here we use a novel experimental design using laser capture microdissection to selectively separately harvest senescent and non-senescent annulus cells in paraffin-embedded tissue, and then compare their gene expression with microarray analysis. An initial in vitro study using cultured human annulus cells was first performed to test whether the

ORGANISM(S): Homo sapiens

SUBMITTER: Helen Gruber 

PROVIDER: E-GEOD-17077 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress