Toward a Better Understanding of Potential Roles of Astrocytes in HIV-1-associated Neurocognitive Disorders

ABSTRACT: We present a microarray analysis of primary mouse astrocytes exposed to HIV-1 in culture. Results are compared with previous genomic studies of HIV-1 effect in human astrocytes and human and macaque brains. Day-two neonatal mouse astrocytes were exposed in triplicates to cell-free HIV-1/NL4-3 at m.o.i. of 1 and cultured for 24 hours. As control, cells were treated in triplicates with a mock virus concentrate at the same dilution (vol/vol) as the wild-type virus. As an example of large scale gene expression analysis we shall provide some details of the method; full description is in Kim et al. (S.-Y. Kim et al., 2004). Total RNA was prepared from cell cultures using the RNAeasy total RNA extraction kit (Qiagen, CA). RNA quality was assessed by electrophoresis and spectrophotometric anal

ORGANISM(S): Mus musculus

SUBMITTER: Alejandra Borjabad 

PROVIDER: E-GEOD-17383 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress