ABSTRACT: Expression data after flg22 treatment on leaf discs in Col-0, 35S:AFB1 and 35S:miR393 We used microarrays to investigated the effect of auxin signalling on flg22 response pathway. We also compared if addition of auxin could simulated the 35S:AFB1 phenotype. Keywords: stress response, disease state analysis Leaf discs were taken and let overnight in water to avoid the wound response. We then performed the treatment (flg22, flg22 from Agrobacterium tumefaciens, flg22 +NAA, NAA) to the media. The samples where harvested and freeze in liquid nitrogen at 0, 1 and 2h after treatment.
Project description:Expression data after flg22 treatment on leaf discs in Col-0, 35S:AFB1 and 35S:miR393 We used microarrays to investigated the effect of auxin signaling on flg22 response pathway. We also compared if addition of auxin could simulated the 35S:AFB1 phenotype. Keywords: stress response, disease state analysis
Project description:Expression data 24hrs after PstDC3000 inoculation in Col-0, 35S:AFB1 and 35S:miR393. We used microarrays to investigated the effect of auxin signaling on PstDC3000 response. Keywords: Alexandre,Robert-Seilaniantz Plants were syringe infiltrated with 10^6cfu/ml of PstDc3000 or water. Leaves were harvested 24hrs after inoculation
Project description:Expression data 24hrs after PstDC3000 inoculation in Col-0, 35S:AFB1 and 35S:miR393. We used microarrays to investigated the effect of auxin signaling on PstDC3000 response. Keywords: Alexandre,Robert-Seilaniantz
Project description:Genome-wide transcriptome analysis of Arabidopsis thaliana was performed to understand the role of auxin in the response of leaf growth to osmotic stress. We studied transcriptional changes in proliferating leaves of the seedlings grown in vitro on control medium, medium supplemented with 25mM mannitol, 0.1μM NAA and 0.1μM NAA + 25mM mannitol.
Project description:The aim of the project is to compare NAA-induced proteome remodelling between wild-type plants (Arabidopsis thaliana, Col-0) to autophagy deficient mutants (atg2-1) treated MS growt media suplemented with solvent (EtOH- Control) or NAA (a synthetic variant of the plant hormone auxin. The aim is to look at the rapid response when treated with auxin and as so the treatment is very short at 30 minutes. The time of the experiment was at zeitgeber 0.
Project description:Leaf explants of the superembryogenic Medicago truncatula line 2HA were treated with auxin (1-naphthaleneacetic acid) for one week to induce the formation of roots (Imin et al J Exp Bot 58:439-451). Gene expression in the leaves and the NAA treated tissue cultures was compared to identify transcripts expressed during the commitment to root formation in tissue culture. We have used the Affymetrix Medicago Genome Array GeneChip to compared gene expression in Medicago truncatula leaves and leaf explants that have been cultured for one week on NAA, to identify genes expressed during the commitment to root formation in tissue culture. Experiment Overall Design: Medicago truncatula 2HA leaves and leaf explants treated with NAA for one week were collected; total RNA was extracted and used for hybridization to Affymetrix arrays
Project description:Here we show that differntiated endodermal cells have a distinct transcriptional response to auxin treatment. We perform a time serie of 10µM NAA treatment and sample at t=0, 2, 4, 8, 16 and 24hrs after NAA treatment. For the time series we compared roots of the solitairy root 1 (slr-1) mutant to the CASP1::shy2-2/slr-1 double mutant. We also provide RNAseq data of slr-1, CASP1::shy2-2 and CASP1::shy2-2/slr-1 at t=0
Project description:Arabidopsis lines expressing 35S::Strep-SUMO1H89R in Col-0 background were generated since the H89R SUMO1 variant simplifies MS/MS detection of SUMO conjugated lysines after trypsinization (Miller et al., 2010). Transgenic seedlings were treated with water or 250nM flg22 for 30 minutes and total protein extracts including membrane fractions were affinity purified with Pierce™ Streptavidin Magnetic Beads. LC-MS analysis of eluting proteins allowed identification of candidate SUMO-conjugated proteins in response to flg22 treatment.
Project description:Leaf explants of the superembryogenic Medicago truncatula line 2HA were treated with auxin (1-naphthaleneacetic acid) for one week to induce the formation of roots (Imin et al J Exp Bot 58:439-451). Gene expression in the leaves and the NAA treated tissue cultures was compared to identify transcripts expressed during the commitment to root formation in tissue culture. We have used the Affymetrix Medicago Genome Array GeneChip to compared gene expression in Medicago truncatula leaves and leaf explants that have been cultured for one week on NAA, to identify genes expressed during the commitment to root formation in tissue culture. Keywords: Cell type comparison