Transcription profiling of mouse spleen from animals exposed to Ebola virus reveals reduced levels of protein tyrosine phosphatase CD45 protect mice from the lethal effects of Ebola virus infection

ABSTRACT: To gain insight into the changes in gene expression pattern upon Ebola infection, CD45+/+ (100% protein level) and CD45+/- (62% protein level) mice were challenged with mouse adapted Ebola virus. At time-points day 0, 1, 3, 5, 7, 9, 11 and 13, spleen tissue was harvested and splenocytes isolated. Total RNA was isolated for mRNA expression analysis. The mouse genome 430 2.0 array (Affymetrix, Inc.), which consists of over 39,000 genes in a single array, was used. Based on gene expression patterns, the variable genes were grouped into sixteen clusters. Each cluster contained genes associated with cellular immune processes, signaling, cell-cycle, complement coagulation cascade, biosynthesis/metabolism, ubiquitous genes involved in several cascades, and genes of unknown function. Interesting

ORGANISM(S): Mus musculus

SUBMITTER: Brian Peyser 

PROVIDER: E-GEOD-17509 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress