Genome changes associated with a Zymomonas mobilis sodium acetate-tolerant mutant (AcR)

ABSTRACT: We report the genome changes associated with a Zymomonas mobilis sodium acetate-tolerant mutant (AcR). We used comparative genomics, transcriptomics, and genetics to show nhaA over-expression conferred sodium acetate (NaAc) tolerance in Z. mobilis. We observed a synergistic effect for sodium and acetate ions that enhanced toxicity against the wild-type strain (ZM4), which was not observed for similar concentrations of potassium and ammonium acetate under controlled laboratory conditions. We extended our studies and demonstrated that Saccharomyces cerevisiae sodium-proton antiporter genes contribute to NaAc tolerance for this important ethanologen. The application of classical and systems biology tools is a paradigm for industrial strain improvement and combines benefits of few a priori ass

ORGANISM(S): Zymomonas mobilis

SUBMITTER: Stan Martin 

PROVIDER: E-GEOD-18106 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress