Microarray analysis of CL-20 reversible neurotoxicity in the earthworm Eisenia fetida

ABSTRACT: The earthworm Eisenia fetida is one of the most used species in standardized soil ecotoxicity tests. Endpoints such as survival, growth and reproduction are ecologically relevant but provide little mechanistic insight into the toxicity pathways, especially at the molecular level. To better understand toxicological modes of action and to facilitate the development of molecular biomarkers, we have obtained 30,245 unique EST sequences from E. fetida and have designed a novel microarray with 15,119 60-mer oligonucleotide probes. These probes target the unique non-redundant EST sequences identified in E. fetida. Using this array we have profiled gene expression of E. fetida after exposure to CL-20, a cage cyclic nitramine previously found exhibiting reversible neurotoxicity to worms. Worms were

ORGANISM(S): Eisenia fetida

SUBMITTER: Xin Guan 

PROVIDER: E-GEOD-18536 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress