Modulation of calcium activated potassium channels induces cardiogenesis of pluripotent stem cells and enrichment of pacemaker- like cells

ABSTRACT: Background: Ion channels are key determinants for the function of excitable cells but little is known about their role and involvement during cardiac development. Earlier work identified Ca2+-activated potassium channels of small and intermediate conductance (SKCas) as important regulators of neural stem cell fate. Here, we have investigated their impact on the differentiation of pluripotent cells towards the cardiac lineage. Methods and Results: We have applied the SKCa-activator EBIO on embryonic stem cells and identified this particular ion channel family as a new critical target involved in the generation of cardiac pacemaker-like cells: SKCa-activation led to rapid remodeling of the actin cytoskeleton, inhibition of proliferation, induction of differentiation and diminished teratoma f

ORGANISM(S): Mus musculus

SUBMITTER: Martin Zenke 

PROVIDER: E-GEOD-18660 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress