Whole-Genome Transcriptional analysis of Sulfate and Sulfonate assimilation by Bradyrhizobium japonicum USDA 110

ABSTRACT: The ability of Bradyrhizobium japonicum and B. elkanii strains to utilize alkane and aromatic sulfonates as sole sources of sulfur for growth was investigated. All of the strains tested were able to utilize alkane sulfonates, but not aromatic sulfonates for growth. Whole-genome transcriptional profiling was used to assess B. japonicum USDA 110 genes involved in growth on alkane sulfonates, as compared to growth on sulfate and cysteine. Two sets of genes, bll7007 to bll7011 and bll6449 to 6456 were highly expressed during growth with sulfate and sulfonates. These genes were predicted to encode alkanesulfonate monooxygenases and ABC transporter components. Reverse transcription-PCR (RT-PCR) analyses showed that these genes were organized in two operon-like structures and expressed as polycis

ORGANISM(S): Bradyrhizobium japonicum

SUBMITTER: Oleg Paliy 

PROVIDER: E-GEOD-19039 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress