Comparative analysis of gene expression in retinal layers

ABSTRACT: Purpose: The aim of this study was to give a comprehensive overview on spatial distribution of gene expression in the adult mouse retina and integrate this information into existing retinal gene expression databases. Methods: Total RNA was collected by laser capture microdissection from the ganglion cell layer, inner nuclear layer, photoreceptors and the retinal pigmented epithelium of adult mice and was analyzed by oligonucleotide microarrays. The results were validated by quantitative real time PCR and in situ hybridization. Results: The applied method resulted in good separation of cells of different retinal layers. The spatial distribution of gene expression was determined on a global scale in the retina and the RPE. Our results show good correlation with previously reported reti

ORGANISM(S): Mus musculus

SUBMITTER: Laszlo Hackler 

PROVIDER: E-GEOD-19304 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress