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Transcription profiling of mouse dendritic cells reveals stimulatory effect of Echinacea purpurea extract on trafficking activity

ABSTRACT: To seek scientific evidence supporting therapeutic use of Echinacea, the transcriptomic effects of Echinacea purpurea extract on mouse bone marrow-derived dendritic cells (BMDCs) were analyzed using primary cultures. Affymetrix DNA microarray and bioinformatics analyses of genes differentially expressed in DCs treated with [BF/S+L/Ep] for 4 or 12 h revealed that the majority of responsive genes were related to cell adhesion or motility (Cdh10, Itga6, Cdh1, Gja1 and Mmp8), or were chemokines (Cxcl2, Cxcl7) or signaling molecules (Nrxn1, Pkce and Acss1). Experiment Overall Design: BMDCs from mice were treated with [BF/S+L/Ep] or DMSO for 4 h or 12 h in duplicates.

ORGANISM(S): Mus musculus

SUBMITTER: Ning-Sun Yang

PROVIDER: E-GEOD-19369 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Stimulatory effect of Echinacea purpurea extract on trafficking activity in mouse dendritic cells

Project description:To seek scientific evidence supporting therapeutic use of Echinacea, the transcriptomic effects of Echinacea purpurea extract on mouse bone marrow-derived dendritic cells (BMDCs) were analyzed using primary cultures. Affymetrix DNA microarray and bioinformatics analyses of genes differentially expressed in DCs treated with [BF/S+L/Ep] for 4 or 12 h revealed that the majority of responsive genes were related to cell adhesion or motility (Cdh10, Itga6, Cdh1, Gja1 and Mmp8), or were chemokines (Cxcl2, Cxcl7) or signaling molecules (Nrxn1, Pkce and Acss1).

2010-09-13 | GSE19369 | GEO

PDL2+, PDL2- and LPS treated PDL2- WT bone-marrow derived murine DCs

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2013-09-17 | E-GEOD-50898 | biostudies-arrayexpress

STING mediates lupus via the activation of conventional dendritic cell maturation and plasmacytoid dendritic cell differentiation

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2020-09-14 | PXD019239 | Pride

Proteomic analysis identifies the potential effects of trichostatin A and bone marrow-derived dendritic cells on NIH3T3 cells under oxygen–glucose deprivation conditions

Project description:Fibroblasts usually mediate acute wound healing and long-term tissue remodeling with scarring in tissue injury. In myocardial infarction (MI), following a prolonged lack of oxygen supply, necrotized cardiomyocytes become replaced by secreted extracellular matrix proteins produced by fibroblasts. Dendritic cells (DCs) act as inflammatory cells and can migrate from the bone marrow to the infarct areas and infarct border areas to mediate collagen accumulation after MI, whereas trichostatin A (TSA) can regulate the apoptosis and proliferation of the fibroblasts and affect DCs functions under oxygen–glucose deprivation (OGD) conditions. In this study, we used proteomics to investigate the effects of TSA and bone marrow-derived dendritic cells (BMDCs) on NIH3T3 fibroblasts under OGD conditions. Results showed that the fatty acid degradation pathway was significantly upregulated in NIH3T3 cells under OGD conditions, and the fatty acid synthesis pathway was significantly downregulated in NIH3T3 cells treated with BMDCs conditioned media with TSA (BMDCs-CM[TSA]) under OGD conditions. Meanwhile, the BMDCs-CM(TSA) significantly decreased the levels of triglycerides and free fatty acids and mediated ten fatty acid metabolism-related proteins in the NIH3T3 cells under OGD conditions. Summarily, the proteomic analysis showed that TSA and BMDCs affect fatty acid metabolism in NIH3T3 cells under OGD conditions.

2024-05-21 | PXD021469 | Pride

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2012-07-22 | E-GEOD-39563 | biostudies-arrayexpress

Transcription profiling of human dendritic cells treated with Echinacea purpurea phytocompounds

Project description:This study investigated the specific and differential gene expression in human immature DCs (iDCs) in response to treatment with a butanol fraction containing defined bioactive phytocompounds extracted from stems and leaves of Echinacea purpurea Experiment Overall Design: Human immature DCs (iDCs) differentiated from primary monocytes in vitro were incubated with [BF/S+L/Ep] or cichoric acid for 4 or 24 h to characterize early- or late-responsive genes. A total of nine Affymetrix HU-133A chips were hybridized to determine the transcriptome profiles in human iDCs.

2008-10-25 | E-GEOD-12259 | biostudies-arrayexpress

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Cross-Platform Validation of Neurotransmitter Release Impairments in Schizophrenia Patient-Derived NRXN1-Mutant Neurons

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2021-03-12 | GSE168762 | GEO

Intellectual disability and mood disorder associated with partial deletion of the Neurexin 1 Gene

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