Silencing factors induce the elimination of nucleosome-free regions in S. pombe heterochromatin

ABSTRACT: Abstract: In the yeast S. pombe, multiple chromatin-modifying enzymes are required for heterochromatin formation, yet how their actions alter chromatin structure to block access to DNAby the transcriptional machinery is unknown. We constructed high-resolution nucleosome occupancy maps of heterochromatic regions in wild-type strains and in mutants lacking the H3K9 methyltransferase Clr4 or one of the two activities of the silencing effector complex SHREC. Fourier analysis reveals that these enzymes do not increase the regularity of nucleosome spacing. Rather, their principal effect was to induce the elimination of nucleosome-free regions (NFRs). Both NFRs associated with transcription initiation sites as well as those not associated with promoters are affected. As in S. cerevisiae, repre

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Hiten Madhani 

PROVIDER: E-GEOD-19596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress