Expression data from human ovarian cancer cells induced by IL-6 and CNTO328

ABSTRACT: The SKOV-3 and Caov-3 ovarian cancer cell lines where seeded onto the 100mm cell culture plate for overnight. The cells were then incubated with IL-6 (30ng/ml) alone for one hour or pre-treated with siltuximab for four hours and then treated with IL-6 for one hour. Total RNA was collected from these cells using TRIzol® Reagent (GIBCO Grand Island, NY) according to the manufacturerâ??s instructions. To account for and eliminate biologic noise, RNA was isolated from three distinct flasks of each cell line and pooled together. RNA quality was determined via ethidium bromide staining following agarose/formaldehyde gel electrophoresis. Total RNA was processed and hybridized to Affymetrix Genechip HG-U95Av2 arrays (Affymetrix, Santa Clara, CA) by the Gene Array Technology Center at Harvard Med

ORGANISM(S): Homo sapiens

SUBMITTER: Zhenfeng Duan 

PROVIDER: E-GEOD-20272 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress