Transcription profiling of human endothelial progenitor cells (EPC) vs. human umbilical vein endothelial cells (HUVEC) vs. CD14+ monocytes

ABSTRACT: Comparison of mRNA expression in human EPC vs. HUVEC vs. human monocytes. Cell-type specific gene expression under basal cell culture conditions (no stimulation). The hybridization was performed with three samples of EPC vs. three samples of HUVEC vs. 3 samples of CD14+ monocytes. â?¢ The origin of the biological sample:; Human endothelial progenitor cells (EPC): EPC were ex vivo cultivated from human peripheral blood-derived mononuclear cells (PBMC). PBMC were isolated by density gradient centrifugation from healthy human volunteers as previously described (Dimmeler et al., 2001). Pooled human umbilical vein endothelial cells (HUVEC) were purchased from Cambrex (Verviers, Belgium). CD14+ monocytes were purified from PBMC by positive selection with anti-CD14-microbeads (Miltenyi Biotec, Be

ORGANISM(S): Homo sapiens

SUBMITTER: Carmen Urbich 

PROVIDER: E-GEOD-2040 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress