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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human CD34 cells isolated from cord blood or peripheral blood transduced with AML1-ETO or empty vector (MIGR1)

ABSTRACT: Human CD34 cells isolated from cord blood or peripheral blood transduced with AML1-ETO or empty vector (MIGR1).

ORGANISM(S): Homo sapiens

SUBMITTER: Agnes Viale

PROVIDER: E-GEOD-2049 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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AML1-ETO fusion protein up-regulates TRKA mRNA expression in human CD34+ cells, allowing nerve growth factor-induced expansion.

Mulloy James C JC Jankovic Vladimir V Wunderlich Mark M Delwel Ruud R Cammenga Jorg J Krejci Ondrej O Zhao Hui H Valk Peter J M PJ Lowenberg Bob B Nimer Stephen D SD

Proceedings of the National Academy of Sciences of the United States of America 20050224 11

The AML1-ETO fusion protein, generated by the t(8;21) in acute myeloid leukemia (AML), exerts dominant-negative functions and a variety of gains of function, including a positive effect on the growth of primary human CD34+ hematopoietic stem/progenitor cells. We now show that AML1-ETO expression up-regulates the level of TRKA mRNA and protein in these cells and that AML1-ETO-expressing CD34+ hematopoietic cells grown in the presence of five early-acting hematopoietic cytokines further proliferat ...[more]

PMID: 15731354

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Gene expression data from human CD34+ cord blood cells infected with MIGR1, MIGR1-AML1-ETO or MIGR1-AML1-ETO∆NHR1

Project description:Compare the gene expression profile among human CD34+ cord blood cells infected with MIGR1, MIGR1-AML1-ETO or MIGR1-AML1-ETO∆NHR1 AML1-ETO promotes the self-renewal of human hematopoietic stem/progenitor cells (HSPCs). We found deletion of NHR1 domain abrogates AML1-ETO induced expasion of HSPCs. GFP+CD34+ human cord blood cells were sorted by FACS 72 hours after the infection for RNA extraction and hybridyzation for Affymetrix microarrays.

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Transcription profiling by array of human cord-blood-derived CD34+ cells activated with cytokines

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662 adult acute myeloid leukemia (AML) cases characterized on the Affymetrix Human Genome U133 Plus 2.0 Microarray

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Transcription profiling by array of human CD19+CD127+ and CD19+CD127- B cell populations derived from neonatal cord blood CD34+ cells

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Receptor tyrosine kinase dependent phospho-signaling networks determine cellular growth and survival in acute lymphoblastic leukemia

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2019-11-22 | PRD000749 | Pride

Gene expression profiles of AML derived stem cells: similarity to hematopoietic stem cells

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2011-12-01 | E-GEOD-34044 | biostudies-arrayexpress

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2010-08-01 | E-MEXP-2757 | biostudies-arrayexpress

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2011-05-25 | E-GEOD-29522 | biostudies-arrayexpress

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