Poly(A)-Specifc Ribonuclease 1 (PARN-1) Overexpression in Procyclic T. brucei

ABSTRACT: We sought to determine the effect of TbPARN-1 overexpression on global mRNA steady-state levels in T. brucei. The mRNA expression profile of tet-induced TbPARN-1 OvEx procyclic cells was compared to the mRNA profile of control cells (expressing tet-induced TAP tag alone) by microarray analysis. Since overexpression of TbPARN-1 showed increased deadenylation activity in cytoplasmic extracts, overexpressing PARN-1 in vivo should result in more rapid deadenylation and decay of mRNAs targeted by PARN-1. Thus, mRNAs with lower steady state levels in PARN-1 OvEx cells can be considered likely targets for PARN-1-dependent deadenylation. Three different clones of the PARN-1 OvEx and Control cell lines were used for analysis. Each experiment was run in duplicate, with the dye-labeling reversed

ORGANISM(S): Trypanosoma brucei

SUBMITTER: Christopher Utter 

PROVIDER: E-GEOD-20593 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress