PTIP promotes chromatin changes critical for immunoglobulin class switch recombination

ABSTRACT: Programmed genetic rearrangements in lymphocytes require transcription at antigen receptor genes to promote accessibility for initiating double-strand break (DSB) formation critical for DNA recombination and repair. Here we show that activated B cells deficient in the PTIP component of the MLL3 (mixed-lineage leukemia 3) /MLL4 complex display impaired histone methylation (H3K4me3) and transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus leading to defective immunoglobulin class-switching. We also show that PTIP accumulation at DSBs contributes to class-switch recombination (CSR) and genome stability independently from Igh switch transcription. These results demonstrate that PTIP promotes specific chromatin changes that control the accessibili

ORGANISM(S): Mus musculus

SUBMITTER: Chongzhi Zang 

PROVIDER: E-GEOD-20852 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress