Effect of blood sample storage on the study of gene expression in response to ionizing radiation exposure

ABSTRACT: For around ten years, microarrays have been suggested for the diagnosis of ionizing radiation exposure. We assessed for the first time the relevance of gene expression profiling in a real accidental case. This study was performed on peripheral blood mononuclear cells of 41 potential victims. The different strategies of analysis highlighted a huge effect of the blood sample handling on the gene expression profiles. This effect was so high that it could mask specific modulations as a potential effect of ionizing radiation exposure. Thus, we assessed a new way of blood sampling adapted to gene expression analysis: PAXgene. With this method, more than 70% of the modulations of gene expression induced 3 hours after an ex vivo exposure to 0.5 Gy were preserved even in a 24-hour delayed analysis

ORGANISM(S): Homo sapiens

SUBMITTER: Gaetan GRUEL 

PROVIDER: E-GEOD-21240 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress