Dynamic single-cell imaging of direct reprogramming reveals an early specifying event

ABSTRACT: The study of induced pluripotency often relies on experimental approaches that average measurements across a large population of cells, the majority of which do not become pluripotent. Here we used high-resolution, time-lapse imaging to trace the reprogramming process over 2 weeks from single mouse embryonic fibroblasts (MEFs) to pluripotency factor-positive colonies. This enabled us to calculate a normalized cell-of-origin reprogramming efficiency that takes into account only the initial MEFs that respond to form reprogrammed colonies rather than the larger number of final colonies. Furthermore, this retrospective analysis revealed that successfully reprogramming cells undergo a rapid shift in their proliferative rate that corresponds to a reduction in cellular area. This event occurs as

ORGANISM(S): Mus musculus

SUBMITTER: Richard Koche 

PROVIDER: E-GEOD-21361 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress